9PN1

Crystal structure of Q108K:K40L:T51V:T53C:R58W:T29L:Y19W:Q4A mutant of cellular retinol binding protein II complex with 15-cis-retinal

Replaces:  7LHJ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-07-12
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.127
Spacegroup name	P 1
Unit cell lengths	30.923, 36.038, 64.026
Unit cell angles	86.20, 86.56, 65.06

Refinement procedure

Resolution	22.320 - 1.260
R-factor	0.1946
Rwork	0.194
R-free	0.22050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.404
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.320	1.310
High resolution limit [Å]	1.260	1.260
Rmeas	0.107	0.291
Rpim	0.060	0.163
Number of reflections	58186	5523
<I/σ(I)>	13	4.33
Completeness [%]	86.2
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	298	30% PEG 4000, 0.1 M sodium acetate, 0.1 M ammonium acetate pH 4.6, evaporation, temperature 298K