Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-06-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979338 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 144.281, 144.281, 144.281 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.010 - 2.330 |
| R-factor | 0.212 |
| Rwork | 0.211 |
| R-free | 0.23440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.104 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.141 | 2.367 |
| High resolution limit [Å] | 2.327 | 2.327 |
| Rmerge | 0.204 | 1.594 |
| Rmeas | 0.219 | 1.697 |
| Rpim | 0.077 | 0.581 |
| Number of reflections | 21594 | 1075 |
| <I/σ(I)> | 6.5 | 1.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.1 | 8.4 |
| CC(1/2) | 0.994 | 0.373 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1 M MES, 1 M sodium citrate, 5 mM TCEP |
