9PME

Crystallographic structure of MazF-E24A toxin bound to SamF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS SIRIUS BEAMLINE MANACA
Synchrotron site	LNLS SIRIUS
Beamline	MANACA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-02-28
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97718
Spacegroup name	P 31 2 1
Unit cell lengths	59.222, 59.222, 66.432
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	51.290 - 1.520
R-factor	0.1714
Rwork	0.170
R-free	0.20250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	1.594
Data reduction software	XDS (BUILT=20230630)
Data scaling software	XDS (BUILT=20230630)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.290	1.590
High resolution limit [Å]	1.520	1.520
Rmeas	0.095	2.048
Number of reflections	21219	2539
<I/σ(I)>	14.23	0.98
Completeness [%]	99.5	96.67
Redundancy	17.6
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.1 M sodium cacodylate pH 6.5, 1.26 M (NH4)2SO4.