9PI5

Poly dA bound form of Single stranded DNA-binding protein(ICP8) from Herpes simplex virus-1. Mutations: C254S, C455S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979330
Spacegroup name	P 32 2 1
Unit cell lengths	151.171, 151.171, 154.561
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	130.918 - 3.000
Rwork	0.200
R-free	0.23770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.353
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0431 (refmacat 0.4.126))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	154.560	3.120
High resolution limit [Å]	3.000	3.000
Rmerge	0.053	0.828
Number of reflections	41340	4617
<I/σ(I)>	11.7	1
Completeness [%]	100.0	100
Redundancy	1.9
CC(1/2)	0.998	0.312

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.02 M magnesium chloride, 0.05 M PIPES, pH 7.5, 4% PEG8000, 0.001 M spermine