9PI0
Crystal structure of the Spo0B-Spo0A complex from Bacillus subtilis (crystal form II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 108 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-11-02 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 221.240, 103.145, 66.461 |
| Unit cell angles | 90.00, 96.91, 90.00 |
Refinement procedure
| Resolution | 28.720 - 3.500 |
| R-factor | 0.245 |
| Rwork | 0.243 |
| R-free | 0.29200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.378 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.720 | 3.690 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmeas | 0.159 | 1.016 |
| Rpim | 0.082 | 0.521 |
| Number of reflections | 18675 | 2660 |
| <I/σ(I)> | 8.3 | 1.7 |
| Completeness [%] | 91.3 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.997 | 0.596 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M sodium fluoride, 20% w/v PEG3350 |
