9PHR

BTB modified at C151 with monobimane

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-21
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9201
Spacegroup name	P 65 2 2
Unit cell lengths	42.976, 42.976, 266.628
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.360 - 1.801
Rwork	0.234
R-free	0.28200
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.360	1.940
High resolution limit [Å]	1.801	1.801
Number of reflections	14489	2786
<I/σ(I)>	12.45
Completeness [%]	99.1
Redundancy	36.7
CC(1/2)	0.999	0.485

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.2 M lithium acetate, 5% glycerol, and 14 to 20% PEG 3350