9PHI

[18-7B AC] 18 bp tensegrity triangle that propagates via blunt-end stacking with A stacking on C at the interface

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.007430
Spacegroup name	I 41 3 2
Unit cell lengths	150.006, 150.006, 150.006
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.540 - 6.020
R-factor	0.1539
Rwork	0.153
R-free	0.15640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.786
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	61.240	61.240	6.669
High resolution limit [Å]	6.020	9.891	6.024
Number of reflections	827	210	209
<I/σ(I)>	26.4
Completeness [%]	100.0
Redundancy	64.5
CC(1/2)	0.999	0.998	0.962

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate