9PHH
TTLL5 CID-RPGR BD Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-08 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.97741 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 62.906, 62.906, 166.631 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.980 - 2.800 |
| R-factor | 0.2607 |
| Rwork | 0.258 |
| R-free | 0.28910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.394 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.980 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.060 | 0.428 |
| Number of reflections | 8879 | 1237 |
| <I/σ(I)> | 36 | |
| Completeness [%] | 100.0 | |
| Redundancy | 25.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM sodium citrate, pH 6.4, 1.625 M lithium sulfate |
