9PG9

Structure of UBR2-AF27 complex

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	29.224, 43.697, 56.671
Unit cell angles	90.00, 102.42, 90.00

Refinement procedure

Resolution	34.296 - 1.220
R-factor	0.1799
Rwork	0.179
R-free	0.19810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.172
Data reduction software	DIALS
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.240
High resolution limit [Å]	1.220	3.310	1.220
Rmerge	0.113	0.060	0.494
Rmeas	0.123	0.066	0.547
Rpim	0.047	0.025	0.230
Number of reflections	40214	2092	1938
<I/σ(I)>	11.5
Completeness [%]	96.8	97.3	95.6
Redundancy	6.6	6.3	5.1
CC(1/2)	0.998	0.997	0.922

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295.15	26% PEG4000, 0.1M HEPES, protein ligand ratio 1 to 10