9PG4

Structure of UBR2-AF23 complex

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	27.798, 43.736, 27.970
Unit cell angles	90.00, 117.39, 90.00

Refinement procedure

Resolution	24.834 - 1.180
R-factor	0.175
Rwork	0.174
R-free	0.18950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.762
Data reduction software	DIALS
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.240
High resolution limit [Å]	1.180	3.310	1.220
Rmerge	0.097	0.053	1.281
Rmeas	0.104	0.057	1.563
Rpim	0.038	0.020	0.866
Number of reflections	47384	2648	1294
<I/σ(I)>	7.2
Completeness [%]	95.3	99.2	53.2
Redundancy	6.8	7.2	2.3
CC(1/2)	0.993	0.998	0.195

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295.15	27% PEG4000, 0.1M HEPES, protein ligand ratio 1 to 2