9PFP

Structure of POU2F3 POU domains bound to coactivator OCA-T1 and DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	49.785, 94.260, 56.801
Unit cell angles	90.00, 115.05, 90.00

Refinement procedure

Resolution	34.760 - 1.700
R-factor	0.1765
Rwork	0.175
R-free	0.20440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.932
Data reduction software	XDS (January 10, 2022)
Data scaling software	pointless (1.12.13)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.760	1.740
High resolution limit [Å]	1.700	1.700
Rmerge	0.040	0.904
Rmeas	0.047	1.085
Rpim	0.025	0.591
Total number of observations	177735
Number of reflections	51249	2642
<I/σ(I)>	13.6
Completeness [%]	99.0	96
Redundancy	3.5	3.2
CC(1/2)	0.998	0.713

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	291	0.1 M MIB (2:3:3 molar ratio of malonate:imidazole:boric acid), pH 4.0, 25% PEG-1500