9PF4

Saccharomyces cerevisiae SRP54 NG domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-03-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9202
Spacegroup name	P 21 21 2
Unit cell lengths	61.064, 158.954, 35.811
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.740 - 2.550
R-factor	0.2194
Rwork	0.214
R-free	0.27140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.877
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21rc1_5156)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.740	2.650
High resolution limit [Å]	2.550	2.550
Rmerge	0.152	0.696
Number of reflections	11989	1271
<I/σ(I)>	9.98
Completeness [%]	100.0
Redundancy	8.13
CC(1/2)	0.996	0.913

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	5% v/v Tacsimate 7.0, 0.1 M HEPES 7.5, 10 % PEG 5000