9PF3

Crystal structure of the glycosyltransferase QsFucT from Quillaja saponaria

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-02
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	55.237, 76.552, 58.975
Unit cell angles	90.00, 115.40, 90.00

Refinement procedure

Resolution	48.150 - 2.110
R-factor	0.2093
Rwork	0.207
R-free	0.23470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.519
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.150	2.180
High resolution limit [Å]	2.110	2.110
Number of reflections	48854	2538
<I/σ(I)>	7.6
Completeness [%]	99.1
Redundancy	3.8
CC(1/2)	0.996	0.320

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M sodium chloride, 0.1 M BIS-Tris pH 5.5 25 % (w/v) PEG 3,350