9PE0
CryoEM structure of the GSDME pore, C27
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | FEI FALCON I (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.64 |
| Software | cryoSPARC,UCSF Chimera,PHENIX |
| Symmetry | C27 |
| CTF correction | PHASE FLIPPING ONLY |
