9PDQ
Human OCTN2 bound to carnitine in the occluded conformation
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.72 |
| Number of Particle | 6410000 |
| Software | cryoSPARC,PHENIX,EPU,ISOLDE,Coot |
| CTF correction | PHASE FLIPPING ONLY |
