9PDO

Arginine-bound CASTOR1 from Homo sapiens (GATOR2-inspired update)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	91.393, 82.601, 96.666
Unit cell angles	90.00, 116.23, 90.00

Refinement procedure

Resolution	86.710 - 1.800
R-factor	0.1826
Rwork	0.182
R-free	0.21260
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.972
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.720	1.850
High resolution limit [Å]	1.800	1.800
Number of reflections	116806
<I/σ(I)>	25.9
Completeness [%]	97.8
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291.15	0.1 M sodium acetate, 0.25 M ammonium acetate, 22.5% PEG 3350