9PDM

Leucine-bound Sestrin2 from Homo sapiens (GATOR2-inspired update)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	I 2 3
Unit cell lengths	293.029, 293.029, 293.029
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	92.660 - 2.700
R-factor	0.1918
Rwork	0.191
R-free	0.22930
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.083
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.670	2.750
High resolution limit [Å]	2.700	2.700
Number of reflections	114283
<I/σ(I)>	24.47
Completeness [%]	100.0
Redundancy	40
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	1.2 M disodium malonate, 0.1 M MES pH 6.0, 1% (v/v) Jeffamine ED 2001