9P9M
CA-SP1 immature lattice assembled in vitro with inhibitor lenacapavir (dialyzed to 50nM)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.93 |
| Number of Particle | 3929347 |
| Software | cryoSPARC,PHENIX,Coot,SerialEM,UCSF ChimeraX |
| Symmetry | C6 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
