9P7Q
273K human S-adenosylmethionine decarboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Collection date | 2025-05-19 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.978, 45.018, 72.061 |
| Unit cell angles | 90.00, 105.11, 90.00 |
Refinement procedure
| Resolution | 34.860 - 2.210 |
| R-factor | 0.2219 |
| Rwork | 0.220 |
| R-free | 0.25880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.098 |
| Data reduction software | xia2 |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.860 | 2.270 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.286 | |
| Rmeas | 0.347 | 2.246 |
| Rpim | 0.192 | 1.220 |
| Number of reflections | 14760 | 1089 |
| <I/σ(I)> | 4.5 | 2 |
| Completeness [%] | 97.4 | 97.6 |
| Redundancy | 3.1 | 3.2 |
| CC(1/2) | 0.942 | 0.340 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 4mg/mL enzyme in 200mM NaCl 50mM HEPES pH 7.5 1:1 to reservoir solution containing 4-8% PEG 8000 and 50mM Tris-HCl pH 8.5 |
