9P7B
The Structure of Bovine Trypsin Complexed With Mellitic Acid at 173 Degrees
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2012-06-06 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 68.235, 68.235, 73.555 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.070 - 1.500 |
| R-factor | 0.212 |
| Rwork | 0.209 |
| R-free | 0.25350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.791 |
| Data reduction software | d*TREK |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.070 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.065 | 0.210 |
| Number of reflections | 31388 | 480 |
| <I/σ(I)> | 14.3 | 1.7 |
| Completeness [%] | 97.6 | |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | Sitting drop vapor diffusion in Cryschem plates. 0.5 Reservoirs of 50% TACSIMATE pH 6. Drops 3.5 ul 40 mg/ml protein stock solution plus 3.5 ul of the reservoir. Room temperature. |
