9P77
Structure of Bovine Trypsin Complexed with Mellitic Acid at Room Temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU R-AXIS |
| Temperature [K] | 292 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-04-16 |
| Detector | RIGAKU AFC9 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 69.200, 69.200, 75.270 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.600 - 1.300 |
| R-factor | 0.1706 |
| Rwork | 0.169 |
| R-free | 0.20880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.749 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.800 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.121 | 0.450 |
| Number of reflections | 43964 | 430 |
| <I/σ(I)> | 13.9 | 2.1 |
| Completeness [%] | 100.0 | |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | vapor diffusion at pH 6.0 with 50% TACSIMATE as reservoir precipitant. 6 ul droplets, 0.50 ml reservoirs. Drops 50% 40 mg/ml protein stock and 50% reservoir. Room temperature |
