9P6I
Crystal Structure of the Histidine Kinase VC2136 from Vibrio cholerae serotype O1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-17 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97848 |
| Spacegroup name | P 31 |
| Unit cell lengths | 86.211, 86.211, 212.636 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.220 - 2.750 |
| R-factor | 0.2425 |
| Rwork | 0.240 |
| R-free | 0.28631 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.845 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.106 | |
| Rpim | 0.034 | 0.418 |
| Number of reflections | 45970 | 2314 |
| <I/σ(I)> | 23.9 | 1.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.7 | 10.9 |
| CC(1/2) | 0.995 | 0.634 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | Protein: 6.5 mg/ml, 0.15M Sodium chloride, 0.01M Tris pH 8.3; Screen: Anions (G8), 0.37M Sodium sulfate, 0.1M MES pH 6.5. Cryo: 2M Lithium sulfate. |
