9P5E

Crystal structure of hen egg white lysozyme at 100 Kelvin with Glycerol 30% (Triplicate)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER TURBO X-RAY SOURCE
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-01
Detector	Bruker PHOTON II
Wavelength(s)	1.54184
Spacegroup name	P 43 21 2
Unit cell lengths	78.483, 78.483, 36.989
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.767 - 1.498
Rwork	0.171
R-free	0.20140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.855
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.767	10.000
High resolution limit [Å]	1.498	1.498
Number of reflections	19073	19073
<I/σ(I)>	18
Completeness [%]	99.7
Redundancy	1
CC(1/2)	0.990	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	295	1.2 M sodium chloride, 100 mM sodium acetate, pH 4.6