9P55

Structure of DNA-free long form XPD from Thermoplasma acidophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.11583
Spacegroup name	P 21 21 21
Unit cell lengths	59.530, 96.000, 159.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.822 - 2.127
Rwork	0.202
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	2.026
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0431 (refmacat 0.4.105))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	159.090	2.180
High resolution limit [Å]	2.110	2.110
Rmerge	0.160	0.540
Number of reflections	52613	3828
<I/σ(I)>	11.2
Completeness [%]	99.1	89.4
Redundancy	12.8	9.8
CC(1/2)	0.999	0.091

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	1M Sodium Potassium tartrate, 0.2M Sodium Chloride, 0.1M bis-Tris Propane pH 7