9P2P
Streptomyces thermoviolaceus ClpC2 C-terminal domain dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE 7B2 |
| Synchrotron site | CHESS |
| Beamline | 7B2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-04 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 47.849, 46.065, 71.281 |
| Unit cell angles | 90.00, 105.53, 90.00 |
Refinement procedure
| Resolution | 34.340 - 1.410 |
| R-factor | 0.1905 |
| Rwork | 0.189 |
| R-free | 0.21410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.487 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.430 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Number of reflections | 28906 | 1415 |
| <I/σ(I)> | 63.78 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6 | |
| CC(1/2) | 0.999 | 0.734 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.75 | 293.15 | 0.1 M Bis-tris, pH 5.75, 29% PEG3350 |
