9P06

Structure of human Sec23a/Sec24a/Sec22b bound to ligand CPD12

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-02
Detector	DECTRIS EIGER R 4M
Wavelength(s)	0.978
Spacegroup name	C 1 2 1
Unit cell lengths	147.782, 95.937, 129.712
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.000 - 2.650
R-factor	0.20828
Rwork	0.206
R-free	0.27450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.390
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.000	2.720
High resolution limit [Å]	2.600	2.650
Rmerge	0.110	0.379
Number of reflections	50419	3636
<I/σ(I)>	8.7
Completeness [%]	99.2
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50 mM Tris, pH 7.8, 0.3 M NaAC, 15% PEG4000