9P03

Structure of human Sec23a/Sec24a/Sec22b bound to CPD6

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-02
Detector	DECTRIS EIGER R 4M
Wavelength(s)	0.978
Spacegroup name	C 1 2 1
Unit cell lengths	148.185, 96.489, 130.801
Unit cell angles	90.00, 90.26, 90.00

Refinement procedure

Resolution	50.000 - 2.640
R-factor	0.2084
Rwork	0.205
R-free	0.26770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.051
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.640
Rmerge	0.082	0.436
Number of reflections	53105	2154
<I/σ(I)>	11.1
Completeness [%]	98.1
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50 mM Tris, pH 7.8, 0.3 M NaAC, 15% PEG4000