9OZL

Crystal structure of the polysaccharide lyase RbmB from Vibrio cholerae

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979338
Spacegroup name	P 1 21 1
Unit cell lengths	118.438, 48.419, 134.897
Unit cell angles	90.00, 109.70, 90.00

Refinement procedure

Resolution	72.500 - 2.100
R-factor	0.198
Rwork	0.197
R-free	0.24210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.505
Data reduction software	MOSFLM
Data scaling software	Aimless (0.7.15)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.500	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.130	1.260
Rpim	0.090	0.900
Number of reflections	84767	4388
<I/σ(I)>	8.2	1.5
Completeness [%]	99.8	99.5
Redundancy	5.1	5.2
CC(1/2)	0.980	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	0.4M Ammonium formate, PEG 3,350