9OZB

Crystal Structure of FosA from Pseudomonas aeruginosa and Bromomethyl Phosphonic Acid

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	55.447, 66.011, 74.941
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.530 - 1.990
R-factor	0.1956
Rwork	0.191
R-free	0.23640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.885
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.550	2.040
High resolution limit [Å]	1.990	1.990
Number of reflections	67323	2767
<I/σ(I)>	11
Completeness [%]	100.0
Redundancy	13.2
CC(1/2)	0.998	0.585

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	40.5% PEP629, 0.075M K2HPO4 (pH 7.0), 40 mM KCl, 1 mM MnCl2