9OZ5
Gradient equilibration of tetragonal lysozyme from 8% NaCl to 3% NaCl over 40 minutes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION NOVA |
| Temperature [K] | 294 |
| Detector technology | CCD |
| Collection date | 2025-06-01 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.182, 79.182, 37.985 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.000 - 1.300 |
| R-factor | 0.1614 |
| Rwork | 0.161 |
| R-free | 0.17470 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.904 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 56713 | 1460 |
| <I/σ(I)> | 20.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 8.9 | |
| CC(1/2) | 1.000 | 0.354 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 8% NaCl, 20 mM NaOAc pH 5.2 |
