9OZ0

Crystal structure of T6SS effector-immunity complex PA3907-PA3908 from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2018-03-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97929
Spacegroup name	P 41 3 2
Unit cell lengths	167.329, 167.329, 167.329
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.580 - 2.871
R-factor	0.2305
Rwork	0.228
R-free	0.28550
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.686
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((dev_3092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.920
High resolution limit [Å]	2.870	2.870
Rmerge	0.097	1.337
Rpim	0.029	0.458
Number of reflections	18909	925
<I/σ(I)>	20.2	1
Completeness [%]	100.0	100
Redundancy	12.2	9.3
CC(1/2)		0.528

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	PEG3350 25%, KCl 0.1M, NaCl 0.1M, MgCl2 10mM, Hepes 0.1M pH 7.5, 10mM NH4Citrate