9OWF

X-ray crystal structure of Zika virus envelope glycoprotein in complex with Fab of F25.S02

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-16
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.976
Spacegroup name	P 41 21 2
Unit cell lengths	117.855, 117.855, 346.096
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.700 - 2.300
R-factor	0.2277
Rwork	0.225
R-free	0.27360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.746
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.700	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.164	1.878
Number of reflections	206051	5312
<I/σ(I)>	20.8	1.7
Completeness [%]	99.9
Redundancy	16.3
CC(1/2)	0.993	0.615

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Tris, pH 8.0, 0.1 M NaCl, 5% w/v PEG 20K