9OVH
Structure of an ancestral ethylene forming enzyme, Anc357, in complex with Mn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-18 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.127 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.588, 87.470, 85.492 |
| Unit cell angles | 90.00, 95.43, 90.00 |
Refinement procedure
| Resolution | 42.550 - 2.150 |
| R-factor | 0.1987 |
| Rwork | 0.197 |
| R-free | 0.22620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.016 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19_4092: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.650 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.160 |
| Number of reflections | 31511 | 2758 |
| <I/σ(I)> | 4.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.993 | 0.572 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 25 % PEG 3350, 100 mM HEPES (pH 7.5), and 200 mM sodium chloride |
