9OV5
C1 symmetry cryoEM structure of the soluble-WRAPed membranous portion of MspA (Mycobacterium smegmatis porin), dimerized along the native interface.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.34 |
| Number of Particle | 7804230 |
| Software | cryoSPARC,UCSF ChimeraX,Coot,PHENIX,ISOLDE,UCSF Chimera |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
