9OUV

Crystal structure of human IGG1 FC fragment-FC-gamma receptor IIB complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-05-16
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	49.385, 76.086, 120.718
Unit cell angles	90.00, 95.53, 90.00

Refinement procedure

Resolution	32.630 - 3.070
R-factor	0.1886
Rwork	0.186
R-free	0.24080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.793
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.120
High resolution limit [Å]	3.070	3.070
Rmerge	0.154	0.794
Rpim	0.094	0.574
Number of reflections	16276	710
<I/σ(I)>	9.9	1.3
Completeness [%]	95.9	84.5
Redundancy	3.4
CC(1/2)	0.950	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	294	150 mM sodium chloride 100 mM Tris-HCl pH 8.0 8% PEG 6000