9OUG

Influenza A Virus Nucleoprotein(8-498)NP complex with rac-5-(4-(((2R,6R)-6-(methoxymethyl)-6-methyl-1,4-dioxan-2-yl)methoxy)phenyl)-2-oxo-6-(trifluoromethyl)-1,2-dihydropyridine-3-carboxamide (Compound 20)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-25
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	60.151, 60.151, 584.428
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.440 - 2.330
R-factor	0.2744
Rwork	0.272
R-free	0.32160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.860
Data reduction software	Adxv
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.11.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.440	2.460
High resolution limit [Å]	2.330	2.330
Rmerge		1.694
Number of reflections	48313	6831
<I/σ(I)>	6.2
Completeness [%]	100.0
Redundancy	11.2
CC(1/2)	0.979	0.424

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	0.1 M Tris, pH 8.5, 22.5% PEG800, 0.1 M magnesium chloride