9OU4

K-Ras Wild Type 1-169 bound to BI-2865 at 313 K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE 7B2
Synchrotron site	CHESS
Beamline	7B2
Temperature [K]	313
Detector technology	PIXEL
Collection date	2025-04-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	P 21 2 21
Unit cell lengths	40.923, 56.444, 86.929
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.340 - 1.890
R-factor	0.1852
Rwork	0.181
R-free	0.22030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.742
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.340	1.950
High resolution limit [Å]	1.890	1.890
Rmeas	0.101
Rpim	0.071	0.187
Number of reflections	16740	784
<I/σ(I)>	19.9	0.9
Completeness [%]	98.7
Redundancy	2
CC(1/2)	0.985	0.875

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PEG2000, 0.2 mM magnesium chloride, 100 mM sodium acetate, pH 4.6