9OU3

K-Ras Wild Type 1-169 bound to BI-2865 at 293K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE 7B2
Synchrotron site	CHESS
Beamline	7B2
Temperature [K]	293
Detector technology	PIXEL
Collection date	2025-04-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9686
Spacegroup name	P 21 2 21
Unit cell lengths	40.489, 55.465, 87.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.780 - 1.500
Rwork	0.231
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.063
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.780	1.760
High resolution limit [Å]	1.500	1.730
Rmeas	0.040	0.440
Rpim	0.016	0.178
Number of reflections	39849	1021
<I/σ(I)>	39.2	1.3
Completeness [%]	98.4
Redundancy	3.4
CC(1/2)	0.993	0.893

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	277	25% PEG2000, 0.2 mM magnesium chloride, 100 mM sodium acetate, pH 4.6