9OTW

Influenza A Virus Nucleoprotein(8-498)NP complex with 5-(4-{[(2R)-6,6-dimethyl-1,4-dioxan-2-yl]methoxy}phenyl)-2-oxo-6-(trifluoromethyl)-1,2-dihydropyridine-3-carboxamide (compound 13)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-16
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	60.173, 60.173, 582.064
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	145.520 - 2.244
R-factor	0.2467
Rwork	0.244
R-free	0.28800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.870
Data reduction software	Adxv
Data scaling software	Aimless
Phasing software	PHASES
Refinement software	BUSTER (2.11.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	145.520	145.520
High resolution limit [Å]	2.240	2.240
Number of reflections	53720	53720
<I/σ(I)>	8.4
Completeness [%]	100.0
Redundancy	11.5
CC(1/2)		0.543

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	0.1 M Tris, pH 8.5, 22.5% PEG800, 0.1 M magnesium chloride