9OTP
Human glutamine synthetase R298A decamer under turnover conditions
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 1.95 |
| Software | cryoSPARC |
| Symmetry | C5 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
