9OSC
Crystal structure of HP1gamma chromoshadow domain in complex with KAP1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-19 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.726, 60.726, 43.042 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.310 - 1.770 |
| R-factor | 0.20795 |
| Rwork | 0.206 |
| R-free | 0.23587 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.758 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.800 |
| High resolution limit [Å] | 1.770 | 4.800 | 1.770 |
| Rmerge | 0.041 | 0.031 | 0.362 |
| Rmeas | 0.043 | 0.032 | 0.390 |
| Rpim | 0.012 | 0.010 | 0.140 |
| Total number of observations | 105578 | ||
| Number of reflections | 9004 | 505 | 388 |
| <I/σ(I)> | 25.8 | ||
| Completeness [%] | 97.0 | 98.8 | 85.7 |
| Redundancy | 11.7 | 10.3 | 6.8 |
| CC(1/2) | 0.999 | 1.000 | 0.939 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1.0 M LiCl2, 0.1 M citrate buffer pH 4.0, and 20% PEG6000 |
