9ORM
The structure of human Vacuolar Protein Sorting 34 catalytic domain bound to RD-I-137
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-11-12 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.979460 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 113.402, 113.402, 145.946 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.370 - 2.060 |
| R-factor | 0.2076 |
| Rwork | 0.206 |
| R-free | 0.22710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.934 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.370 | 2.134 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.096 | 3.689 |
| Rmeas | 3.756 | |
| Rpim | 0.019 | 0.704 |
| Number of reflections | 59359 | 5811 |
| <I/σ(I)> | 22.56 | 1.01 |
| Completeness [%] | 99.8 | |
| Redundancy | 27.3 | |
| CC(1/2) | 1.000 | 0.441 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 298 | 0.1 M Ammonium acetate, 0.1 M BIS-TRIS pH 5.5, 17% w/v Polyethylene glycol 10,000 |
