9OPF

Context-Dependent Variability Of HIF Heterodimers Influences Interactions With Macromolecular And Small Molecule Partners

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-05
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9201
Spacegroup name	C 1 2 1
Unit cell lengths	263.114, 23.826, 31.851
Unit cell angles	90.00, 91.82, 90.00

Refinement procedure

Resolution	29.020 - 2.280
R-factor	0.2693
Rwork	0.266
R-free	0.29510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.654
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419-000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.020	2.400
High resolution limit [Å]	2.280	2.280
Rmerge	0.085	0.630
Number of reflections	9247	1264
<I/σ(I)>	7
Completeness [%]	98.4
Redundancy	3.3
CC(1/2)	0.992	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.1 M imidazole, 50% (+/-)-2-Methyl-2,4-pentanediol (MPD)