9OP9

Two Component Protein Nano-Particle (T=3). De Novo Design, Computationally Relaxed into Low Resolution Subtomogram Averaged CryoEM Map with Icosahedral Symmetry Applied

This is a non-PDB format compatible entry.

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	TFS GLACIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	200
Cs (mm)	2.7
Energy Filter	GIF Bioquantum
Detector	GATAN K3 (6k x 4k)

3D Reconstruction

Method	SUBTOMOGRAM AVERAGING
Resolution (Å)	31.6
Number of Particle	142674
Software	cryoSPARC,EMAN2,SerialEM,Rosetta,PHENIX
Symmetry	I
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION