Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-08-24 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.181 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 64.640, 65.670, 128.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.070 - 2.250 |
| R-factor | 0.2197 |
| Rwork | 0.215 |
| R-free | 0.26530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.505 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.070 | 2.300 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmeas | 0.067 | 2.880 |
| Number of reflections | 26660 | 1663 |
| <I/σ(I)> | 24.91 | 1.13 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13 | 13.4 |
| CC(1/2) | 1.000 | 0.593 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 200 mM ammonium sulfate, 100 mM HEPES pH 7.5, 35% poly (acrylic acid sodium salt) 2100 |
