9OML
WrtF fucosyltransferase - acetylated trisaccharide acceptor
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-09 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.150, 122.350, 45.590 |
| Unit cell angles | 90.00, 93.52, 90.00 |
Refinement procedure
| Resolution | 43.070 - 1.900 |
| R-factor | 0.1954 |
| Rwork | 0.194 |
| R-free | 0.23320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.842 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.500 | 1.968 |
| High resolution limit [Å] | 1.610 | 1.900 |
| Number of reflections | 60682 | 3674 |
| <I/σ(I)> | 4.9 | |
| Completeness [%] | 99.6 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.994 | 0.442 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 200 mM ammonium sulfate, 100 mM HEPES pH 7.5, 35% poly (acrylic acid sodium salt) 2100 |
