9OLI
Crystal structure of the melibiose-bound melibiose transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 126.421, 126.421, 103.555 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.700 - 3.050 |
| R-factor | 0.2589 |
| Rwork | 0.255 |
| R-free | 0.29370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.545 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER (1.21.1_5286) |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.260 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.055 | |
| Rmeas | 0.067 | |
| Rpim | 0.031 | |
| Number of reflections | 18439 | 18439 |
| <I/σ(I)> | 11.8 | |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 5.7 | |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 296.15 | Tris-HCl, 100 mM, PH 8.5, NaCl, 100 mM, CaCl, 50 mM, PEG400, 34% |
