9OL2
RNA-imidazolium-bridged dinucleotide intermediate complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 99 |
| Detector technology | PIXEL |
| Collection date | 2021-10-05 |
| Detector | DECTRIS EIGER2 X CdTe 16M |
| Wavelength(s) | 1.033175 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 49.592, 49.592, 81.941 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.948 - 1.528 |
| Rwork | 0.198 |
| R-free | 0.23450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 3.041 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.560 |
| High resolution limit [Å] | 1.528 | 1.528 |
| Rmerge | 0.079 | 0.419 |
| Rmeas | 0.087 | 0.464 |
| Rpim | 0.035 | 0.203 |
| Number of reflections | 17783 | 757 |
| <I/σ(I)> | 15.9 | 2.8 |
| Completeness [%] | 96.9 | 84.9 |
| Redundancy | 5.9 | 4.8 |
| CC(1/2) | 0.979 | 0.860 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10% w/v Polyethylene glycol 6,000, 0.05 M HEPES pH 7.0, 0.2 M Ammonium acetate, 0.15 M Magnesium acetate |
