9OL1

16mer self-complementary duplex RNA with dI:s(2)C pair sequence 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	99
Detector technology	PIXEL
Collection date	2025-03-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97933
Spacegroup name	H 3 2
Unit cell lengths	40.898, 40.898, 124.979
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.077 - 1.243
Rwork	0.186
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	2.079
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.077	1.265
High resolution limit [Å]	1.243	1.243
Rmerge	0.128	2.000
Rmeas	0.135	2.140
Rpim	0.044	0.754
Number of reflections	11727	572
<I/σ(I)>	12.5	2
Completeness [%]	100.0	100
Redundancy	17.6	14.7
CC(1/2)	0.997	0.439

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.2 M Calcium chloride dihydrate, 0.05 M HEPES sodium pH 7.5, 28% v/v Polyethylene glycol 400, 0.002 M Spermine