9OL0
16mer self-complementary duplex RNA with dI:s(2)C pair sequence 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 99 |
| Detector technology | PIXEL |
| Collection date | 2025-03-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97933 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 41.293, 41.293, 124.404 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.370 - 1.150 |
| R-factor | 0.2057 |
| Rwork | 0.203 |
| R-free | 0.23310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.773 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.370 | 1.170 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.061 | 1.256 |
| Rmeas | 0.068 | 1.434 |
| Rpim | 0.029 | 0.677 |
| Number of reflections | 14617 | 819 |
| <I/σ(I)> | 14.9 | 1.5 |
| Completeness [%] | 98.1 | 94.5 |
| Redundancy | 9.8 | 8.2 |
| CC(1/2) | 0.999 | 0.431 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M Magnesium chloride hexahydrate, 0.1 M HEPES sodium pH 7.5, 30% v/v Polyethylene glycol 400 |
